Validating dynex

The increase in the detection of fentanyl has provided the impetus to have a rapid, selective and sensitive screening procedure for both blood and urine specimens.The use of enzyme-linked immunosorbent assay (ELISA) and other immunoassays for the rapid screening of blood and urine specimens is quite common, but there have not been any publications reporting a validation following the guidelines proposed by the Scientific Working Group for Forensic Toxicology (SWGTOX) (8).Fentanyl ready-to-use enzyme-linked immunosorbent assay kit was validated following the Scientific Working Group for Forensic Toxicology Standard Practices for Method Validation in Forensic Toxicology Laboratory Guidelines.Two decision points, 0.5 and 1 ng/m L, were successfully validated for whole blood.Fentanyl has four common forms when prescribed: oral transmucosal lozenges, effervescent buccal tablets, transdermal patches and injectable formulations (fentanyl citrate) (3).Recently, illicit fentanyl that has similar color and texture to heroin has been substituted for, or added to heroin (4).

Two decision points are incorporated in the methods used in this laboratory.Due to its high potency, fentanyl is used to treat pain for patients who regularly receive other opioids and have acquired opioid tolerance; these patients may have breakthrough pain that is associated with cancer or have chronic pain (3).As an opioid, fentanyl has similar pharmacological and central nervous system depressant effects as other opioids like morphine and oxycodone.Abuse has escalated in the last several years with illicit use rising and fentanyl analogs becoming increasingly popular as well (5).This has corresponded to an increase in the incidence of fentanyl in forensic specimens (6, 7).

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