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Patients receiving incompatible blood transfusion may have pale skin, splenomegaly, hepatomegaly and the yellowing of mouth and eyes.

In addition, their urine may appear in dark colour and the patients may experience dizziness and confusion.

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Blood samples can be obtained through venipuncture of the mother.The method of extracting foetal DNA from maternal blood plasma is considered to be a type of non-invasive prenatal testing.However, in the case of maternal Rh D status being negative, invasive prenatal testing may be used to determine the foetal Rh D status instead. By checking the existence of the Rh D gene in the individual’s genome, the presence of rhesus D (Rh D) antigens can be inferred.Individuals with a positive Rh D status has Rh D antigens expressed on the cell membrane of their red blood cells, whereas Rhesus D antigens are absent for individuals with negative Rh D status.

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However, if the mother were to conceive another Rh D child in the future, the anti-Rh D antibodies will cross the placenta to attack and lyse the red cells of the foetus, causing the aforementioned haemolytic reaction in the foetus known as haemolytic disease of the newborn.

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